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Validation of

Youfen Xu, Zong Li, Ruyin Liu, Hongxia Liang, Zhisheng Yu, Hongxun Zhang

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 4, doi: 10.1007/s11783-020-1246-9

Abstract: -1-BacTaqMan, and Pig-2-BacTaqMan MST assays showed better performance. • The pig-specific MST assaysWe aimed to compare the performance of five previously reported pig-specific MST assays (PF, Pig-Bac1SYBR, Pig-Bac2SYBR, Pig-1-BacTaqMan, and Pig-2-BacTaqMan, which are based on Bacteroidales 16S rRNA geneHowever, the two SYBR green qPCR assays (Pig-Bac1SYBR and Pig-Bac2SYBR) cross-reacted with most non-pigOur results regarding the specificity of these pig-specific MST assays differ from those reported in

Keywords： Microbial source tracking     Pig fecal pollution     16S rRNA gene markers     Pig-specific <i>Bacteroidalesi>    

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<i>RR1i> and <i>RR2i> gene deletion affects the immunogenicity of a live attenuated pseudorabiesvirus vaccine candidate in natural pig host

Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1,   Pages 81-86 doi: 10.15302/J-FASE-2016088

Abstract: In an animal study, the virus could elicit humoral immune responses shown by generation of gB-specificprotection against virulent pseudorabies virus challenge since vaccinated pigs showed clinical pseudorabies-specific

Keywords： pseudorabies virus     <i>RR1i> and <i>RR2i>     ribonucleotide reductase     vaccine candidate    

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First Isolation and Characterization of <i>Ochrobactrum anthropii> from Pig Article

Shijiang Gu, Ruiqing Hou, Shengguo Gao, Zhe Sun, Xiangdong Li, Lufeng Zhai, Yunyun Jin, Qiaoyan Zhu, Yonghong Liao, Kegong Tian

Engineering 2020, Volume 6, Issue 1,   Pages 49-55 doi: 10.1016/j.eng.2019.08.014

Abstract:

One Gram-negative bacillus was isolated from a brain sample of a pig with neurological symptoms.This is the first report on O. anthropi isolated from a pig, and indicates that O. anthropi

Keywords： <i>Ochrobactrum anthropii>     Pig brain    

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Process stability and microbial community composition in pig manure and food waste anaerobic co-digesters

Conor Dennehy, Peadar G. Lawlor, Gillian E. Gardiner, Yan Jiang, Paul Cormican, Matthew S. McCabe, Xinmin Zhan

Frontiers of Environmental Science & Engineering 2017, Volume 11, Issue 3, doi: 10.1007/s11783-017-0923-9

Abstract: reducing hydraulic retention times (HRTs) from 21 days to 10.5 days when anaerobically co-digesting pigReducing HRT from 21 days to 15 days caused a decrease in specific methane yields and VS removal rates

Keywords： Biogas     Sequencing     <i>Clocamonaceaei>     <i>Spiorchatetesi>     Isobutyrate     Biosafety    

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Application of StrucGP in medical immunology: site-specific -glycoproteomic analysis of macrophages

Frontiers of Medicine 2023, Volume 17, Issue 2,   Pages 304-316 doi: 10.1007/s11684-022-0964-8

Abstract: The structure of <i>Ni>-glycans on specific proteins can regulate innate and adaptive immunity viaMeanwhile, the structural diversity of <i>Ni>-glycans poses analytical challenges that limit the explorationof specific glycosylation functions.In this work, we used THP-1-derived macrophages as examples to show the vast potential of a <i>Ni>-glycan structural interpretation tool StrucGP in <i>Ni>-glycoproteomic analysis.

Keywords： macrophage     glycoproteome     glycopeptides     <i>Ni>-glycan structures     PD-L1    

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Identification of genome regions and important variations associated with expression level of

Ye HOU,Lu JING,Yunxia ZHAO,Sheng WANG,An LIU,Shuhong ZHAO,Xinyun LI

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3,   Pages 237-241 doi: 10.15302/J-FASE-2015057

Abstract: Meat quality is one of the most important economic traits in pig breeding.Type II muscle fibers contain three isoforms, IIa, IIb and IIx, which contain specific myofibrillar proteins

Keywords： pig     IMF     pH     <i>MYH1i>     <i>MYH2i>     <i>MYH4i>    

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Cytochrome P450 monooxygenase specific activity reduction in wheat

Kangxin HE,Qixing ZHOU

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 2,   Pages 270-275 doi: 10.1007/s11783-015-0813-y

Abstract: Results indicated that the specific activity of cytochrome P450 (CYP450) monooxygenase was decreasedOn the contrary, the growth related endpoints (i.e., the germination percentage, the biomass and theheight), the content related endpoints (i.e., soluble protein content and CYP450 content), and the superoxideFurther analysis revealed that the CYP450 monooxygenase specific activity reduction was enzymatic mechanism

Keywords： roxithromycin     toxic effect     cytochrome P450 monooxygenase     soil environment     <i>Triticum aestivumi>     biomarker    

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Detection and monitoring prostate specific antigen using nanotechnology approaches to biosensing

Grant Perry, Fernando Cortezon-Tamarit, Sofia I. Pascu

Frontiers of Chemical Science and Engineering 2020, Volume 14, Issue 1,   Pages 4-18 doi: 10.1007/s11705-019-1846-8

Abstract: The detection of the prostate specific antigen (PSA) in blood samples has been the preferred method for

Keywords： biosensing     immunosensor     PSA     prostate cancer     fluorescence    

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<i>NES1i>/<i>KLK10i> and <i>hNISi> gene therapy enhanced iodine-131 internal radiation in PC3 proliferation

Jiajia Hu, Wenbin Shen, Qian Qu, Xiaochun Fei, Ying Miao, Xinyun Huang, Jiajun Liu, Yingli Wu, Biao Li

Frontiers of Medicine 2019, Volume 13, Issue 6,   Pages 646-657 doi: 10.1007/s11684-018-0643-y

Abstract: , we supposed to have an “enhanced firepower” effect by combining overexpressed gene therapy and Itomography/computed tomography (micro-PET/CT) imaging showed that the combined gene therapy and Ithat via inhibition of expression, overexpressed might enhance the effect of radiation therapy of I

Keywords： androgen-independent prostate cancer     normal epithelial cell-specific 1/kallikrein 10     sodium/iodide symporter    

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Transcriptomic basis of neutrophil ratio variation induced by poly I:C stimulation in porcine peripheral

Haiyan WANG, Qiaoxia ZHANG, Lilin YIN, Xiangdong LIU, Shuhong ZHAO, Mengjin ZHU, Changchun LI

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 3,   Pages 342-352 doi: 10.15302/J-FASE-2017162

Abstract: Neutrophils are vital components of defense mechanisms against invading pathogens and are closely linked with the individual antiviral capacity of pigs and other mammals. Neutrophilia is a well-known clinical characteristic of viral and bacterial infections. Using Affymetrix porcine genome microarrays, we investigated the gene expression profiles associated with neutrophil variation in porcine peripheral blood before and after polyriboinosinic-polyribocytidylic acid stimulation. Transcriptomic analysis showed 796 differentially expressed genes (DEGs) in extreme response (ER) pigs and 192 DEGs in moderate response (MR) pigs. Most DEGs were related to immune responses, included , , , , , , , and . Gene ontology analysis indicated that the DEGs of both ER and MR pigs were involved in common biological processes, such as cell proliferation, growth regulation, immune response, inflammatory response and cell activation. The ER and MR groups also showed differences in DEGs involved in biological processes. DEGs involved in cell division and cell cycle were specifically found in the ER pigs, whereas DEGs involved in cell migration were specifically found in the MR pigs. The study provides a basic understanding of the molecular basis for the antiviral capacity of pigs and other mammals.

Keywords： neutrophil     peripheral blood     pig     poly I:C     transcriptome    

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Molecular authentication of the traditional Chinese medicine Tongren Dahuoluo Wan and its alternative formulation

Jikun WANG, Jing DU, Meng CAO, Lu YAO, Suhua XIE, Jiafu CHEN, Xingbo ZHAO

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 3,   Pages 353-357 doi: 10.15302/J-FASE-2017157

Abstract: polymorphic sites of , and genes were screened in leopard and zokor bones, and nine pairs of species-specificcomplex medicine, and successfully confirmed leopard or zokor content using the nine pairs of species-specific

Keywords： Tongren Dahuoluo Wan     molecular diagnostic assay     <i>Eospalax baileyii>     <i>Panthera pardusi>     species-specific    

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Genome-wide search for candidate genes determining vertebrae number in pigs

Longchao ZHANG, Jingwei YUE, Xin LIU, Jing LIANG, Kebin ZHAO, Hua YAN, Na LI, Lei PU, Yuebo ZHANG, Huibi SHI, Ligang WANG, Lixian WANG

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 3,   Pages 327-334 doi: 10.15302/J-FASE-2017163

Abstract: Longer porcine carcasses may be expected to have more vertebrae. Therefore, vertebrae number in pigs is an economically important trait. To examine the genetic basis of this trait, we genotyped 578 F Large White × Minzhu pigs using the Porcine SNP60K BeadChip. A genome-wide association study (GWAS) identified 36 significant single nucleotide polymorphisms (SNPs) on the chromosomes SSC1 (294.28–300.32 Mb) and SSC7 (102.22–109.39 Mb). A 6.04-Mb region that contained all 13 significant SNPs on SSC1 also contained the gene , previously reported to influence the number of vertebrae in pigs. However, the reported putative casual mutation of c.748C>T showed no genome-wide significant association with the trait, suggesting it was not a causal mutation in our population. The remaining 23 significant SNPs on SSC7 were concentrated in a 7.17-Mb region, which was within a quantitative trait locus interval for number of vertebrae. was the closest gene to the most significant SNP and might be a candidate. Haplotype sharing and block analysis refined the QTL to an interval of about 3 Mb containing 29 candidate genes. Of these 29 genes, the previously reported possible casual mutation of g.19034A>C was not found to be a causal mutation in our population. Exploration of these genes via additional genetic and functional studies in mammals revealed that could be a good candidate on SSC7. A mutation of c.1749G>A was detected by GWAS and could be proposed as a candidate causal mutation, or as closely linked to a causal mutation, for the number of vertebrae in pigs.

Keywords： genome-wide association study     number of vertebrae     pig     <i>SSC7i>     <i>TGFβ3i>    

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Development of a new method for RMR and Q classification method to optimize support system in tunneling

Asghar RAHMATI,Lohrasb FARAMARZI,Manouchehr SANEI

Frontiers of Structural and Civil Engineering 2014, Volume 8, Issue 4,   Pages 448-455 doi: 10.1007/s11709-014-0262-x

Abstract: Rock mass classification system is very suitable for various engineering design and stability analysis. classification method is confirmed by Japan Highway Public Corporation that this method can figure out either strength or deformability of rock mass, further appropriating the amount of rock bolts, thickness of shotcrete, and size of pitch of steel ribs just after the blasting procedure. Based on these advantages of method, in this study, according to data of five deep and long tunnels in Iran, two equations for estimating the value of method from and classification systems were developed. These equations as a new method were able to optimize the support system for and classification systems. From classification and its application in these case studies, it is pointed out that the method for the design of support systems in underground working is more reliable than the and classification systems.

Keywords： <i>JHi> classification     <i>Qi> and <i>RMRi> classification     new method    

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The Potential Role of Powdery Mildew-Resistance Gene <i>Pm40i> in Chinese Wheat-Breeding Programs inthe Post-<i>Pm21i> Era Perspective

Shengwen Tang,Yuting Hu,Shengfu Zhong,Peigao Luo

Engineering 2018, Volume 4, Issue 4,   Pages 500-506 doi: 10.1016/j.eng.2018.06.004

Abstract:

Powdery mildew, which is caused by Blumeria graminis f. sp. tritici (Bgt), is an important leaf disease that affects wheat yield. Powdery mildew-resistance (Pm) gene Pm21 was first transferred into wheat in the 1980s, by translocating the Heuchera villosa chromosome arm 6VS to the wheat chromosome arm 6AL (6VS·6AL). Recently, new Bgt isolates that are virulent to Pm21 have been identified in some wheat fields, indicating that wheat breeders should be aware of the risk of deploying Pm21, although pathological details regarding these virulent isolates still remain to be discovered. Pm40 was identified and mapped on the wheat chromosome arm 7BS from several wheat lines developed from the progenies of a wild cross between wheat and Thinopyrum intermedium. Pm40 offers a broad spectrum of resistance to Bgt, which suggests that it is likely to provide potentially durable resistance. Cytological methods did not detect any large alien chromosomal segment in the wheat lines carrying Pm40. Lines with Pm40 and promising agronomical traits have been released by several wheat-breeding programs in the past several years. Therefore, we believe that Pm40 will play a role in powdery mildew-resistance wheat breeding after Pm21 resistance is overcome by Bgt isolates. In addition, both Pm21 and Pm40 were derived from alien species, suggesting that the resistance genes derived from alien species are potentially more durable or effective than those identified from wheat.

Keywords： Wheat     Powdery mildew     <i>Pm21i>     <i>Pm40i>     Alien species     Native resistance    

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NiFeRuO<i>xi> nanosheets on Ni foam as an electrocatalyst for efficient

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 11,   Pages 1698-1706 doi: 10.1007/s11705-023-2334-8

Abstract: The electrocatalyst NiFeRuO<i>xi>/NF, comprised of NiFeRuO<i>xi> nanosheetsNiFeRuO<i>xi>/NF displays high electrocatalytic activity and stability for overall alkalineA solar-driven system containing such a bifunctional NiFeRuO<i>xi>/NF has an almost 100%The NiFeRuO<i>xi> coating around Ni foam is an anti-corrosion layer and also a critical

Keywords： NiFeRuO<i>xi> nanosheets     Ni foam     electrocatalysis     overall seawater splitting     solar-driven    

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Title Author Date Type Operation

Validation of

Youfen Xu, Zong Li, Ruyin Liu, Hongxia Liang, Zhisheng Yu, Hongxun Zhang

Journal Article

<i>RR1i> and <i>RR2i> gene deletion affects the immunogenicity of a live attenuated pseudorabiesvirus vaccine candidate in natural pig host

Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN

Journal Article

First Isolation and Characterization of <i>Ochrobactrum anthropii> from Pig

Shijiang Gu, Ruiqing Hou, Shengguo Gao, Zhe Sun, Xiangdong Li, Lufeng Zhai, Yunyun Jin, Qiaoyan Zhu, Yonghong Liao, Kegong Tian

Journal Article

Process stability and microbial community composition in pig manure and food waste anaerobic co-digesters

Conor Dennehy, Peadar G. Lawlor, Gillian E. Gardiner, Yan Jiang, Paul Cormican, Matthew S. McCabe, Xinmin Zhan

Journal Article

Application of StrucGP in medical immunology: site-specific -glycoproteomic analysis of macrophages

Journal Article

Identification of genome regions and important variations associated with expression level of

Ye HOU,Lu JING,Yunxia ZHAO,Sheng WANG,An LIU,Shuhong ZHAO,Xinyun LI

Journal Article

Cytochrome P450 monooxygenase specific activity reduction in wheat

Kangxin HE,Qixing ZHOU

Journal Article

Detection and monitoring prostate specific antigen using nanotechnology approaches to biosensing

Grant Perry, Fernando Cortezon-Tamarit, Sofia I. Pascu

Journal Article

<i>NES1i>/<i>KLK10i> and <i>hNISi> gene therapy enhanced iodine-131 internal radiation in PC3 proliferation

Jiajia Hu, Wenbin Shen, Qian Qu, Xiaochun Fei, Ying Miao, Xinyun Huang, Jiajun Liu, Yingli Wu, Biao Li

Journal Article

Transcriptomic basis of neutrophil ratio variation induced by poly I:C stimulation in porcine peripheral

Haiyan WANG, Qiaoxia ZHANG, Lilin YIN, Xiangdong LIU, Shuhong ZHAO, Mengjin ZHU, Changchun LI

Journal Article

Molecular authentication of the traditional Chinese medicine Tongren Dahuoluo Wan and its alternative formulation

Jikun WANG, Jing DU, Meng CAO, Lu YAO, Suhua XIE, Jiafu CHEN, Xingbo ZHAO

Journal Article

Genome-wide search for candidate genes determining vertebrae number in pigs

Longchao ZHANG, Jingwei YUE, Xin LIU, Jing LIANG, Kebin ZHAO, Hua YAN, Na LI, Lei PU, Yuebo ZHANG, Huibi SHI, Ligang WANG, Lixian WANG

Journal Article

Development of a new method for RMR and Q classification method to optimize support system in tunneling

Asghar RAHMATI,Lohrasb FARAMARZI,Manouchehr SANEI

Journal Article

The Potential Role of Powdery Mildew-Resistance Gene <i>Pm40i> in Chinese Wheat-Breeding Programs inthe Post-<i>Pm21i> Era

Shengwen Tang,Yuting Hu,Shengfu Zhong,Peigao Luo

Journal Article

NiFeRuO<i>xi> nanosheets on Ni foam as an electrocatalyst for efficient

Journal Article